Ronaldo appeared in an Instagram video shortly after the civil lawsuit was filed on Friday, calling it "fake news".
Ronaldo on his part has vehemently denied the allegations which emerged in German magazine Der Spiegel.
"My clear conscious [sic] will thereby allow me to await with tranquillity [sic] the results of any and all investigations", said Ronaldo in a second Wednesday morning tweet.
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The Nevada woman has also sued Ronaldo and is participating in the investigation, according to a complaint filed by her lawyer.
When Mayorga met Ronaldo she was an aspiring model. Fake, fake news. They want to promote [themselves by using] my name. It's normal. They want to be famous - to say my name.
Cristiano Ronaldo has once again denied the rape allegations made against him and insists that he has a clear conscience.
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Save The Children, a charity that Ronaldo has represented since 2016, expressed its concern at Der Spiegel's contested claims on Tuesday. "This is an inadmissible reporting of suspicions in the area of privacy", lawyer Christian Schertz said in a statement. "I'm a happy man, I'm all good". Mayorga's lawyer did not follow up with Las Vegas Police, according to the lawsuit. The lawsuit said Mayorga also went to a hospital, where a sexual assault medical examination was conducted.
Yesterday it emerged that Las Vegas police have re-opened the investigation into the alleged se'xual assault.
The lawsuit, which seeks more than $200,000 in damages, names as defendants Ronaldo and an unnamed team of fixers described as "personal reputation protection specialists" hired to make the situation go away.
Ronaldo joined Juventus from Real Madrid for £99.2m earlier this year. The Italian club declined to comment on the Der Spiegel report. She filed a lawsuit on September 27 in Clark County District Court in Nevada that has 11 claims, including for battery, abuse of a vulnerable person and intentional infliction of emotional distress.
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